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68724 Sigma-Aldrich

Fast Green FCF

analytical standard

Synonym: Food green 3

  • CAS Number 2353-45-9

  • Linear Formula C37H34N2O10S3Na2

  • Molecular Weight 808.85

  •  Beilstein Registry Number 5718212

  •  Colour Index Number 42053

  •  E Number E143

  •  EC Number 219-091-5

  •  MDL number MFCD00013053

  •  PubChem Substance ID 329761116

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Description

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
BQ4425000

Documents

Certificate of Analysis

Protocols & Articles

Articles

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

Related Products

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