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70184 Sigma-Aldrich

Antibiotic Broth

for microbiology

Synonym: Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth

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Properties

Related Categories A - M, All Liquid Media/Broths, Clinical Media, Escherichia coli & Coliforms, Klebsiella,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   dextrose, 1.0 g/L
  dipotassium hydrogen phosphate, 3.68 g/L
  meat extract, 1.5 g/L
  peptone (from meat), 5.0 g/L
  potassium dihydrogen phosphate, 1.32 g/L
  sodium chloride, 3.5 g/L
  yeast extract, 1.5 g/L
final pH   7.0±0.2 (25 °C)
suitability   nonselective for Escherichia coli and Coliforms
  nonselective for Klebsiella
  nonselective for Staphylococcus
  nonselective for Streptococcus

Description

Application

Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Price and Availability


Sterility Testing
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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