70184 Sigma-Aldrich

Antibiotic Broth

for microbiology

Synonym: Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth



Related Categories A - M, All Liquid Media/Broths, Clinical Media, Escherichia coli & Coliforms, Klebsiella,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   dextrose, 1.0 g/L
  dipotassium hydrogen phosphate, 3.68 g/L
  meat extract, 1.5 g/L
  peptone (from meat), 5.0 g/L
  potassium dihydrogen phosphate, 1.32 g/L
  sodium chloride, 3.5 g/L
  yeast extract, 1.5 g/L
final pH   7.0±0.2 (25 °C)
suitability   nonselective for Escherichia coli and Coliforms
  nonselective for Klebsiella
  nonselective for Staphylococcus
  nonselective for Streptococcus



Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Sterifil Aseptic System and Holder
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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