70195 Sigma-Aldrich

Vogel-Johnson Agar

for microbiology

Synonym: VJ Agar



Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Clinical Media, Control Media for Fish,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 16.0 g/L
  dipotassium hydrogen phosphate, 5.0 g/L
  L-glycine, 10.0 g/L
  lithium chloride, 5.0 g/L
  D-mannitol, 10.0 g/L
  peptone (from casein), 10.0 g/L
  phenol red, 0.025 g/L
  yeast extract, 5.0 g/L
final pH   7.1±0.2 (25 °C)
suitability   selective and differential for Staphylococcus



Vogel and Johnson enhanced the fermentation reaction by increasing the mannitol concentration to 1% (w/v), clearly indicated by the development of yellow zones surrounding the colonies. S. aureus is able to reduce tellurite to metallic tellurium resulting in growth of black colonies. Used for the detection of coagulase-positive and mannitol-utilizing Staphylococci, recommended by USP.

Other Notes

Screening media for detection of methicillin-resistant Staphylococcus aureus from non-sterile body sites

Preparation Note

Supplement: 2 vial/l of sterile 1% potassium tellurite (Cat. No. 17774).

Price and Availability

Sterility Testing

Sterifil Aseptic System and Holder
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Staphylococcus aureus: a Spreading Bacteria

Staphylococcus aureus is frequently a part of our skin flora but is also a cause of a broad range of illnesses. Current studies report a remarkable increase of Methicillin Resistant Staphylococcus au...
Jvo Siegrist
AnalytiX Volume 10 Article 2
Keywords: Aerobic, Antibiotics, Clinical, Environmental, Fermentation, Food & Beverage, Microbiology, Respiration

Peer-Reviewed Papers


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