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70722 Sigma-Aldrich

HiCrome E. coli Agar B

for microbiology

Synonym: E. coli Agar B HiCrome

Purchase

Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Escherichia coli & Coliforms,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  bile salt mixture, 1.5 g/L
  casein enzymic hydrolysate, 20 g/L
  X-glucuronide, 0.075 g/L
final pH   7.2±0.2 (25 °C)
suitability   selective and differential for Escherichia coli and Coliforms
  selective and differential for Klebsiella

Description

Application

HiCrome E. coli Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Reconstitution

Suspend 36.6 g per liter in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Legal Information

HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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