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73009 Sigma-Aldrich

HiCrome ECC Agar

for microbiology

Synonym: E. coli Coliform Agar HiCrome, ECC Agar HiCrome

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Enterobacter,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  chromogenic mixture, 20.3 g/L
  disodium hydrogen phosphate, 3.5 g/L
  lactose, 2.5 g/L
  monopotassium hydrogen phosphate, 1.5 g/L
  neutral red, 0.03 g/L
  peptone, special, 5 g/L
  sodium chloride, 5 g/L
  yeast extract, 3 g/L
final pH   6.8±0.2 (25 °C)
suitability   nonselective and differential for Enterobacter
  nonselective and differential for Escherichia coli and Coliforms
  nonselective and differential for Klebsiella

Description

Application

HiCrome ECC Agar is a differential medium recommended for the presumptive identification of Escherichia coli and other coliforms in food and environmental samples. The chromogenic mixture containstwo chromogens as X-glucuronide and Salmon-GAL. X-glucuronide is cleaved by the enzyme beta glucuronidase produced by Escherichia coli. Salmon-GAL is cleaved by the enzyme galactosidase produced by majority of coliforns, including Escherichia coli. Color of E. coli colonies: blue/purple

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Reconstitution

Suspend 55.8 g in 1000 mL distilled water. Boil gently to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile petri plates.

Legal Information

HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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