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75554 Sigma-Aldrich

Aminopeptidase Test

for microbiology

Purchase

Properties

Related Categories Discs, Strips and Cards, Identification Tests & Reagents, Microbiology More...
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
storage temp.   2-8°C

Description

Components

50 Test Strips (the reaction zone of each strip contains 0.5 μmol L-alanine-4-nitroanilide and buffering agents)

Application

For the detection of L-alanine-aminopeptidase in microorganisms.
L-Alanine aminopeptidase is an enzyme localized in the bacterial cell wall which cleaves N-terminal L-alanine from various peptides. It is found almost exclusively in Gram-negative microorganisms. Studied Gram-positive or Gram-variable microorganisms show no or very weak activity. The aminopeptidase test is a reliable method for determining the Gram behavior. It does not replace Gram-staining, as it cannot show morphology.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and even can be killed by exposure...
Jvo Siegrist
AnalytiX Volume 7 Article 2
Keywords: Aerobic, Anaerobic, Clinical, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology, Pharmaceutical, Vitamins

Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
ivo.siegrist@sial.com
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

EZ-Fit™ Filtration Products for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

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