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77187 Sigma-Aldrich

Peptone Water, phosphate-buffered

for microbiology

Synonym: Buffered Peptone Water, Tryptone Phosphate Water

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Properties

Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Bacteria (General Media), Biochemical Identification Media by Test,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   disodium hydrogen phosphate, 3.5 g/L
  peptone, 10 g/L
  potassium dihydrogen phosphate, 1.5 g/L
  sodium chloride, 5 g/L
final pH   7.2±0.2 (25 °C)
suitability   Providencia
  nonselective for Bacteria (General Media)
  nonselective for Citrobacter
  nonselective for Cronobacter spp.
  nonselective for Enterobacteriaceae
  nonselective for Escherichia coli and Coliforms
  nonselective for Klebsiella
  nonselective for Proteus
  nonselective for Pseudomonas
  nonselective for Salmonella
  nonselective for Shigella
  nonselective for Staphylococcus
  nonselective for Streptococcus
  nonselective for Yersinia

Description

Other Notes

Studies with salmonellae

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Chronobacter spp. Classic and New Detection Methods

Cronobacter is a rod-shaped, motile and facultatively-anaerobic bacteria of the family Enterobacteriaceae. Originally Cronobacter spp. were listed as yellow-pigmented Enterobacter cloacae (see Table ...
Jvo Siegrist
Microbiology Focus Edition 1.2
Keywords: Absorption, Anaerobic, Diagnostic, Environmental, Enzyme-linked immunosorbent assay, Food Safety, Genetic, Microbiology, Nutrition, Polymerase chain reaction, Proteomics, Vitamins

EZ-Product Family for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration, Separation

Enterobacteriaceae in Food

From our library of Articles, Sigma-Aldrich presents Enterobacteriaceae in Food

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Diagnostic, Phosphorylations

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

Peer-Reviewed Papers
15

References

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