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77339 Sigma-Aldrich

Pertussis toxin from Bordetella pertussis

Synonym: Histamine-sensitizing factor, IAP, Islet Activating Protein, PTX, Pertussigen

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Properties

storage temp.   −20°C
Gene Information   Bordetella pertussis Tohama I ... ptxA(2665068), ptxB(2665069), ptxC(2665408)

Description

Other Notes

Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins; Pertussis toxin-catalyzed ADP-ribosylation of G proteins

Physical form

aseptically packaged solution in 50% (v/v) glycerol; 50 mM TRIS, pH 7.5; 10 mM glycine; 0.5 M NaCl

Application

The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al.

Biochem/physiol Actions

Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3462 6.1 / PGI
WGK Germany 
3
RTECS 
XW5883750

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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P7208 Pertussis toxin from Bordetella pertussis, lyophilized powder

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