• USA Home
  • 81938 - Coliform ChromoSelect Agar

EMAIL THIS PAGE TO A FRIEND
81938 Sigma-Aldrich

Coliform ChromoSelect Agar

for microbiology

Synonym: Coliform Agar ChromoSelect

Purchase

Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Citrobacter,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 12 g/L
  chromogenic mixture, 0.2 g/L
  dipotassium hydrogen phosphate, 3 g/L
  peptone, special, 3 g/L
  potassium dihydrogen phosphate, 1.7 g/L
  sodium chloride, 5 g/L
  sodium lauryl sulfate, 0.1 g/L
  sodium pyruvate, 1 g/L
  tryptophan, 1 g/L
final pH   6.8±0.2 (25 °C)
suitability   selective and differential for Citrobacter
  selective and differential for Enterobacter
  selective and differential for Enterobacteriaceae
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Klebsiella
  selective and differential for Salmonella
  selective and differential for Shigella

Description

Preparation Note

Supplement: 5 mg/l Novobiocin (Cat. No. 74675)

Reconstitution

Suspend 27 g in 1 liter distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121 °C for 15 minutes. When a high number of Gram-positive accompanying bacteria are expected, add 5 mg/L Novobiocin (Catalog No. 74675) before autoclaving of the medium.

Application

Coliform ChromoSelect Agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli and total coliforms in water and food samples.

General description

Coliform ChromoSelect Agar is a chromogenic mixture which contains two chromogenic substrates, Salmon-GAL and X-glucuronide. Coliforms produce an enzyme called β-D-galactosidase, which cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by Escherichia coli cleaves X-glucuronide. Escherichia coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction, thereby increasing detection reliability in combination with the two chromogens. To confirm Escherichia coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?