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82897 Sigma-Aldrich

Vitamin B12 Assay Medium

for microbiology



Related Categories All Food Control Media (alphabetical), All Liquid Media/Broths, Lactobacillus, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
pH   5.6±0.2 (25 °C)
suitability   nonselective for Lactobacillus



Composition (per liter):
D(+)-Glucose anhydrous 40 g
Casein hydrolysate "Vitamin-free" 15 g
L-Asparagine 200 mg
L-Cysteinium chloride 200 mg
L-Cystine 400 mg
DL-Tryptophan 400 mg
Adenine 20 mg
Guanine 20 mg
Uracil 20 mg
Xanthine 20 mg
4-Aminobenzoic acid 2 mg
L(+)-Ascorbic acid 4 g
D(+)-Biotin (vitamin H) 10 μg
Calcium D(+)-pantothenate 1 mg
Folic acid 200 μg
Nicotinic acid 2 mg
Pyridoxal hydrochloride 4 mg
Pyridoxine hydrochloride 4 mg
Pyridoxamine hydrochloride 800 μg
Riboflavin 1 mg
Thiaminium dichloride 1 mg
Potassium phosphate dibasic 1 g
Iron(II)sulfate 20 mg
Potassium phosphate monobasic 1 g
Magnesium sulfate 400 mg
Manganese(II) sulfate 20 mg
Sodium acetate anhydrous 20 g
Sodium chloride 20 mg


For the microbiological assay of vitamins in drugs, foodstuffs, animal feed preparations and other materials.

Preparation Note

Dissolve 83 G in 1 L distilled water. To be added: Tween® 80 (Cat. No. 93780) 2 ml.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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