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83339 Sigma-Aldrich

HiCrome MacConkey-Sorbitol Agar

for microbiology

Synonym: MacConkey-Sorbitol Agar HiCrome, SMAC Agar HiCrome, Sorbitol MacConkey Agar HiCrome

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Enterobacteriaceae,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 13.5 g/L
  B.C. indicator, 0.1 g/L
  bile salts mixture, 1.5 g/L
  casein enzymic hydrolysate, 17 g/L
  crystal violet, 0.001 g/L
  neutral red, 0.03 g/L
  proteose peptone, 3 g/L
  sodium chloride, 5 g/L
  sorbitol, 10 g/L
final pH   7.1±0.3 (25 °C)
suitability   selective and differential for Enterobacteriaceae
  selective and differential for Enterovirulent E. coli
  selective and differential for Escherichia coli and Coliforms

Description

Application

HiCrome MacConkey Sorbitol Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β-D-glucuronidase which is specific for E. coli. Stranins of E. coli possessing β-D-glucuronidase appear as blue colored colonies on the medium. Enteropathogenic strains of E. coli 0157 do not possess beta-D-glucuronidase activity and thus produce colorless colonies. E. coli fermenting sorbitol and possessing beta-D-glucuronidase activity produce purple colored colonies. Most of the gram-positive organisms are inhibited by crystal violet and bile salts. Addition of Tellurite-Cefixime Supplement makes the medium selective. Potassium Tellurite selects the serogroups 0157 from other E. coli serogroups and inhibits Aeromonas species and Providencia species. Cefixime inhibits Proteus species.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplement: 2 vial/l Tellurite-Cefixime Supplement (Cat. No. 77981)

Reconstitution

Suspend 50.1 g in 1 liter of distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50 °C. Mix well and pour into sterile petri plates. If desired add Tellurite-Cefixime Supplement to the molten and cooled medium (50 °C) before pouring into sterile petri plates.

Legal Information

HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.

Price and Availability

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Detection of enterovirulent Escherichia coli

E. coli are Gram-negative, rod-shaped bacteria belonging to the family of Enterobacteriaceae. Normally a useful organism in the human digestive system, a few E. coli strains are capable of causing hu...
Jvo Siegrist
AnalytiX Volume 8 Article 4
Keywords: Clinical, Environmental, Indicators, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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