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85927 Sigma-Aldrich

HiCrome ECC Selective Agar

for microbiology

Synonym: E. coli-Coliform Selective Agar HiCrome, ECC Selective Agar HiCrome



Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Citrobacter,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 10.0 g/L
  casein enzymic hydrolysate, 3.3 g/L
  chromogenic mixture, 0.43 g/L
  disodium hydrogen phosphate, 1 g/L
  peptone, special, 6.0 g/L
  sodium chloride, 2 g/L
  sodium dihydrogen phosphate, 0.6 g/L
  sodium pyruvate, 1 g/L
  sorbitol, 1 g/L
7, 0.15 g/L
  tryptophan, 1 g/L
final pH   6.8±0.2 (25 °C)
suitability   selective and differential for Citrobacter
  selective and differential for Enterobacter
  selective and differential for Enterobacteriaceae
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Salmonella
  selective and differential for Shigella



HiCrome ECC Selective Agar is a selective medium recommended for the simultaneous detection of Escherichia coli and coliforms in water and food samples. The ingredients help even the sublethally injured coliforms to grow rapidly, Tergitol inhibits Gram-positive as well as some Gram-negative bacteria other than coliforms. The chromogenic mixture contains two chromogenic substrates as Salmon-GAL and X-glucuronide. The enzyme β-D-galactosidase produced by coliforms cleaves Salmon-GAL, resulting in the salmon-to-red coloration of coliform colonies. The enzyme β-D-glucuronidase produced by E. coli, cleaves X-glucuronide. E. coli forms dark blue-to-violet colored colonies due to cleavage of both Salmon-GAL and X-glucuronide. The addition of tryptophan improves the indole reaction. To confirm E. coli, add a drop of Kovac′s reagent (Catalog No. 60983) on the dark blue-to-violet colony. Formation of cherry-red color indicates the positive reaction.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplement: 5-10 mg/l Cefsulodin (Cat. No. 22126)


Suspend 26.5 g in 1 liter of distilled water. Heat in a boiling water bath or in a free flowing steam, with stirring to dissolve the medium completely (approximately 35 minutes). Medium may show haziness, but it does not affect the performance. If desired to inhibit Pseudomonas and Aeromonas species, add 5 mg of cefsulodin for surface and pour plate method and 10 mg of cefsulodin for membrane filter technique. Mix well.

Legal Information

HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.

Tergitol is a trademark of Dow Chemical Company

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Overview of Chromogenic Media

The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology

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