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860867

Brilliant Cresyl Blue ALD

Certified by the Biological Stain Commission

Synonym(s):

3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C

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About This Item

Empirical Formula (Hill Notation):
C17H20ClN3O · 0.5ZnCl2
CAS Number:
51716-96-2
Molecular Weight:
385.96
Beilstein/REAXYS Number:
5690713
EC Number:
279-675-0
MDL number:
MFCD00148901
UNSPSC Code:
12171500
PubChem Substance ID:
24888521
NACRES:
NA.47

Key Documents

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Quality Level

200

form

powder or crystals

composition

Dye content, ≥50%

technique(s)

microbe id | staining: suitable

mp

233-236 °C (lit.)

λmax

622 nm

ε (extinction coefficient)

≥20000 at 237-241 nm in 50% ethanol at 0.005 g/L
≥20000 at 248-252 nm in 50% ethanol at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.CC\[N+](CC)=C1\C=CC2=Nc3cc(C)c(N)cc3OC2=C1.CC\[N+](CC)=C4\C=CC5=Nc6cc(C)c(N)cc6OC5=C4

InChI

1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

SIHASHFMDFXGSX-UHFFFAOYSA-J

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1 of 4

This Item
B5388B7920B0149
Brilliant Cresyl Blue ALD Certified by the Biological Stain Commission

Sigma-Aldrich

860867

Brilliant Cresyl Blue ALD

Brilliant Cresyl Blue ALD certified by the BSC

Sigma-Aldrich

B5388

Brilliant Cresyl Blue ALD

Brilliant Blue R pure

Sigma-Aldrich

B7920

Brilliant Blue R

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder or crystals

form

powder

form

powder

form

powder

composition

Dye content, ≥50%

composition

Dye content, ≥60%

composition

-

composition

Dye content, ~50%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Brilliant cresyl blue ALD has been used for the staining of reticulocytes.[1]

Biochem/physiol Actions

Brilliant cresyl blue ALD is responsible for the staining of RNA in reticulocytes.[2]

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS7555
SHBR3592
SHBS0804
SHBR5385
SHBP9883

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Inflammatory and oxidative stress phenotypes in transgenic sickle cell mice.
Charrin E, et al.
Blood Cells, Molecules and Diseases, 62, 13-13 (2016)
Pal GK and Pal P
Textbook Of Practical Physiology (2006)
Clark, G., Ed.
Staining Procedures, 176-176 (1981)
A Zh Baltabekova et al.
Molecular biotechnology, 57(8), 746-755 (2015-04-04)
Immunoglobulin E (IgE) plays a central role in type I hypersensitivity including allergy and asthma. Novel treatment strategy envisages development of a therapeutic vaccine designed to elicit autologous blocking antibodies against the IgE. We sought to develop an IgE-epitope antigen
Azza A G Tantawy et al.
Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis, 26(4), 419-425 (2015-02-24)
Endothelial nitric oxide synthase (eNOS), an enzyme that generates nitric oxide, is a major determinant of endothelial function. Several eNOS gene polymorphisms have been reported as 'susceptibility genes' in various human diseases states, including cardiovascular, pulmonary and renal diseases. We
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