86348 Sigma-Aldrich


for microbiology

Synonym: Vibrio Selective Agar



Related Categories All Food Control Media (alphabetical), All Solid Media/Agars, Control Media for Fish, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 14 g/L
  bromothymol blue, 0.04 g/L
  ferric citrate, 1 g/L
  mixed peptone, 10 g/L
  ox-bile, 5 g/L
  sodium chloride, 10 g/L
  sodium cholate, 3 g/L
  sodium citrate, 10 g/L
  sodium thiosulfate, 10 g/L
  sucrose, 20 g/L
  thymol blue, 0.04 g/L
  yeast extract, 5 g/L
final pH   8.6±0.2 (25 °C)
suitability   selective and differential for Vibrio


Other Notes

Incidence of Vibrio cholerae from estuaries of the United States West Coast; Survival and multiplication of Vibrio cholerae serotype Ogawa in reconstituted infant milk


Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.

General description

Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater. Vibrios are facultative aerobe a...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Dehydration reaction, Diagnostic, Fermentation, Indicators, Metabolism, Microbiology

Peer-Reviewed Papers


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