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87898 Sigma-Aldrich

HiCrome MS.O157 Agar

for microbiology

Synonym: MS.O157 Agar HiCrome



Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Enterovirulent E. coli,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 12.00 g/L
  bile salt mixture, 1.00 g/L
  chromogenic mixture, 0.731 g/L
  peptone, special, 10.00 g/L
  sorbitol, 4.00 g/L
pH   6.8±0.2 (25 °C)
suitability   selective and differential for Enterovirulent E. coli
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Klebsiella



Suspend 27.7 g in 1000 mL distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C. Mix well and pour into sterile petri plates. This medium can be made more selective by adding aseptically 0.25 mL of sterile 1% Potassium tellurite solution (Cat. No.17774) to 1 litre molten and cooled medium (45°C).


HiCrome MS.O157 Agar is a selective medium recommended for the simultaneous detection of Escherichia coli, Escherichia coli O157:H7 and coliforms in water and food samples. Escherichia coli O157:H7 gives colorless colonies because of non-fermentation of sorbitol and absence of β-glucuronidase activity, whereas other strains of Escherichia coli having β-glucuronidase activity and fermenting sorbitol appear as steel blue colored colonies. In absence of tellurite various non Escherichia coli O157:H7 may have some colony color. Addition of tellurite makes medium much more specific and selective.

Legal Information

HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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