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88597 Sigma-Aldrich

Hydrogen peroxide solution

3%, for microbiology

Synonym: Catalase Test

  • CAS Number 7722-84-1

  • Empirical Formula (Hill Notation) H2O2

  • Molecular Weight 34.01

  •  Beilstein Registry Number 3587191

  •  MDL number MFCD00011333

  •  PubChem Substance ID 24888972

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Properties

Related Categories Biochemical Reagents for Identification, Identification Tests & Reagents, Microbiology More...
grade   for microbiology
vapor pressure   23.3 mmHg ( 30 °C)
InChI Key   MHAJPDPJQMAIIY-UHFFFAOYSA-N
assay   2.9-3.1% (redox titration)
shelf life   limited shelf life, expiry date on the label
concentration   1-5%
mp   0.0 °C
density   1.000 g/cm3

Description

General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

EZ-Fit™ Filtration Products for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

On the Trail of Campylobacter

The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis1,2. However,...
Jvo Siegrist
AnalytiX Volume 8 Issue 1
Keywords: Antibiotics, Cancer, Citric Acid Cycle, Gram stain, Metabolism, Microbiology, Scanning electron microscopy

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Staphylococcus aureus: a Spreading Bacteria

Staphylococcus aureus is frequently a part of our skin flora but is also a cause of a broad range of illnesses. Current studies report a remarkable increase of Methicillin Resistant Staphylococcus au...
Jvo Siegrist
AnalytiX Volume 10 Article 2
Keywords: Aerobic, Antibiotics, Clinical, Environmental, Fermentation, Food & Beverage, Microbiology, Respiration

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Diagnostic, Phosphorylations

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

Peer-Reviewed Papers
15

References

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