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91230 Sigma-Aldrich

Trichloroacetic acid

ACS reagent, for the determination of Fe in blood according to Heilmeyer, ≥99.5%

Synonym: TCA

  • CAS Number 76-03-9

  • Linear Formula Cl3CCOOH

  • Molecular Weight 163.39

  •  Beilstein Registry Number 970119

  •  EC Number 200-927-2

  •  MDL number MFCD00004177

  •  PubChem Substance ID 24889460

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Properties

Related Categories Acids, Acids & Bases, Acids and Bases, Analytical Reagents, Analytical Reagents for General Use,
grade   ACS reagent
vapor density   <1 (vs air)
vapor pressure   1 mmHg ( 51 °C)
InChI Key   YNJBWRMUSHSURL-UHFFFAOYSA-N
assay   ≥99.5% (T)
  ≥99.5%
quality   for the determination of Fe in blood according to Heilmeyer
ign. residue   ≤0.03% (as SO4)
refractive index   n20/D 1.62(lit.)
bp   196 °C(lit.)
mp   54-58 °C(lit.)
solubility   water: soluble81.7 g/L at 20 °C
density   1.62 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤10 mg/kg
  nitrate (NO3-): ≤20 mg/kg
  phosphate (PO43-): ≤5 mg/kg
  sulfate (SO42-): ≤200 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid may be used in the following studies:
• Estimation of phosphorus in various biological samples (serum, urine and stool ash solutions).
• Elimination of acid-soluble and lipid phosphorous during the determination of deoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues.
• Preparation of 2-methyl-4-nitroanilinium trichloroacetate trichloroacetic acid (2M4NATCA) crystals.

10% Trichloroacetic acid may be employed for the preparation of radioactive proteins.

Other Notes

Protein denaturant

General description

Trichloroacetic acid (TCA) is a chlorinated acetic acid. It is one of the major metabolites of trichloroethylene (TCE).

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 1839 8 / PGII
WGK Germany 
2
RTECS 
AJ7875000
Flash Point(F) 
>235.4 °F
Flash Point(C) 
>113 °C
Protocols & Articles

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