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93283 Sigma-Aldrich

Tris-EDTA buffer solution

BioUltra, for molecular biology, pH 8.0

Synonym: TE buffer solution

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
InChI Key   VLEIUWBSEKKKFX-UHFFFAOYSA-N
product line   BioUltra
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   8.0±0.1 (25 °C)
cation traces   Al: ≤1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   neat
UV absorption   λ: 260 nm Amax: 0.04
  λ: 280 nm Amax: 0.01

Description

Other Notes

Same product — same quality — now Sigma-Aldrich brand

This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8.0 may also be used as a washing buffer.

Price and Availability


Interactive Buffer Table

Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

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