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93321 Sigma-Aldrich

TRIS Glycine buffer solution

BioUltra, 10× concentrate

Synonym: Blotting buffer solution

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Buffer Solutions More...
product line   BioUltra
quality   10× concentrate
composition   glycine, 1.92 M
  TRIS, 0.25 M
impurities   insoluble matter, passes filter test
  proteases, none detected
ign. residue   ≤0.2%
pH   8.5-8.7
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  NH4+: ≤200 mg/kg
  Na: ≤500 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   neat
UV absorption   λ: 260 nm Amax: 0.07
  λ: 280 nm Amax: 0.05

Description

Other Notes

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

Same product — same quality — now Sigma-Aldrich brand

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Price and Availability


Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
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