EMAIL THIS PAGE TO A FRIEND
93349 Sigma-Aldrich

Trizma® base

BioUltra, ≥99.8% (T)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

Purchase

Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
description   aminopeptidase substrate
product line   BioUltra
assay   ≥99.8% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.01% (as SO4)
loss   ≤0.5% loss on drying, 110 °C
pH   10.5-12.0 (25 °C, 4 M in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
  168-172 °C
solubility   H2O: soluble4 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤5 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   4 M in H2O
UV absorption   λ: 260 nm Amax: 0.10
  λ: 280 nm Amax: 0.08

Description

Other Notes

In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis; Electrophoresis of proteins on cellulose acetate membranes; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

This product has been replaced by 93362-SIGMA | Trizma base BioUltra, for molecular biology, ≥99.8% (T)

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
TY2900000
Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?