93745 Sigma-Aldrich

TSC Agar

for microbiology

Synonym: Tryptose Sulfite Cycloserine Agar



Related Categories All Food Control Media (alphabetical), All Solid Media/Agars, Clinical Media, Clostridia, Control Media for Fish,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  ammonium ferric citrate, 1 g/L
  meat peptone (peptic), 15 g/L
  sodium disulfite, 1 g/L
  soya peptone, 5 g/L
  yeast extract, 5 g/L
final pH   7.6±0.2 (25 °C)
suitability   selective and differential for Clostridia



For the isolation and enumeration of vegetative forms as well as spores from Clostridium perfringens in food, clinical specimens and other material acc. to Harmon et al. (1971).

Other Notes

Isolation and enumeration of sulphite-reducing Clostridium spores and Group D faecal streptococci from oysters ; Membrane filtration enumeration of faecal clostridia and C. perfringens in water

Preparation Note

TSC Agar:
400 mg/l D-cycloserine (Cat. No. 30020)
or 2 vial/l Perfringens T.S.C. Supplement (Cat. No. P9352)
and 50 ml/l Egg Yolk Emulsion (Cat. No. 17148)

TSC Agar egg yolk free:
400 mg/l D-cycloserine (Cat. No. 30020)
or 2 vial/l Perfringens T.S.C. Supplement (Cat. No. P9352)

TSC Fluorescence Agar:
5.5 g/l rehydrated TSC Agar supplement (Cat. No. 80548)

SFP Agar (Shahidi-Ferguson Perfringens Agar):
2 vial/l of Perfringens S.F.P. Supplement (Cat. No. P9477)
and 50ml/l Egg Yolk Emulsion (Cat. No. 17148)

Price and Availability

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers


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