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95273 Sigma-Aldrich

VRB MUG Agar

for microbiology

Synonym: Violet Red Bile Lactose MUG Agar

Purchase

Properties

Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Control Media for Fish, Enterobacteriaceae,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 13 g/L
  bile salt mixture, 1.5 g/L
  crystal violet, 0.002 g/L
  lactose, 10 g/L
  meat peptone, 7 g/L
  4-methylumbelliferyl-β-D-glucuronide, 0.1 g/L
  neutral red, 0.03 g/L
  sodium chloride, 5 g/L
  yeast extract, 3 g/L
final pH   7.4±0.2 (25 °C)
suitability   selective and differential for Enterobacteriaceae
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Klebsiella
  selective and differential for Salmonella
  selective and differential for Shigella
  selective and differential for Yersinia

Description

Application

Selective medium for the detection and enumeration of coliform bacteria, in particular E. coli. Gram-positive accompanying flora are extensively inhibited by crystalviolet and bile salts. A color-change to red indicates lactose-positive colonies, within which E. coli can be demonstrated by fluorescence in the UV.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Differentiation and Identification Media for Salmonella

Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry i...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Biochemistry, Fermentation, Food & Beverage, Indicators, Microbiology

Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

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