• USA Home
  • A0701 - Agarose, low gelling temperature

EMAIL THIS PAGE TO A FRIEND
A0701 Sigma-Aldrich

Agarose, low gelling temperature

Type VII-A

Synonym: 2-Hydroxyethyl agarose

Purchase

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting, Life Science Reagents for Nucleic Acid Purification,
type   Type VII-A
impurities   ≤7% water
ash   ≤0.4%
turbidity   ≤4 NTU (1.5% gel)
EEO   ≤0.12
mp   ≤65 °C ( 1.5% gel)
transition temp   gel point 26 °C ±2 °C
gel strength   ≥250 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.4%

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Excellent for in-gel enzymatic reactions and cloning assays and for recovery of heat-labile samples after electrophoresis

Packaging

25, 100 g in poly bottle

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Agarose Product Information

CAS Number: 9012-36-6 (product A9539) CAS Number: 39346-81-1 (products A9414, A4018, A6560, A9045, A0701) Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; ...
Keywords: Cell culture, Cloning, Counterimmunoelectrophoresis, Diffusion, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Indicators, Isoelectric focusing, Melting, Molecular biology, Polymerase chain reaction, Purification, Sample preparations, Separation

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?