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A2790 Sigma-Aldrich

Agarose

Wide range, for molecular biology

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Properties

Related Categories Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,
grade   for molecular biology
EEO   ≤.15
mp   ≤65 °C
transition temp   gel point ≤35 °C
anion traces   sulfate (SO42-): ≤0.15%
foreign activity   DNAse, RNAse, NICKase, none detected

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Wide range agarose is capable of separating DNA fragments from 50-1,000 bp on a single 3% agarose gel.

Packaging

10, 25, 100, 500 g in poly bottle

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

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