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A3648 Sigma-Aldrich

(3-Aminopropyl)triethoxysilane

≥98%

Synonym: 3-Triethoxysilylpropylamine, APTES, APTS

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Properties

Related Categories Additional Reagents, Adhesives, Cell Biology, Chemical Synthesis, Core Bioreagents,
InChI Key   WYTZZXDRDKSJID-UHFFFAOYSA-N
assay   ≥98%
form   liquid
bp   217 °C/760 mmHg(lit.)
density   0.946 g/mL at 25 °C(lit.)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

100, 500 mL in glass bottle

Application

• (3-Aminopropyl)triethoxysilane has been used for the preparation of cadherin surfaces on glass slides.
• It has been used for the preparation of RGD (Arg-Gly-Asp)-treated silicone membranes.
• It has been used for the preparation of the avidin–biotin coverslips.

Biochem/physiol Actions

(3-Aminopropyl)triethoxysilane forms aminopropyl derivative of glass, an adsorbent for affinity chromatography. It is used to prepare positively charged slides suitable for use with various immunohistochemical and in situ hybridization procedures.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2735PSN1 8 / PGII
WGK Germany 
1
RTECS 
TX2100000
Flash Point(F) 
199.4 °F
Flash Point(C) 
93 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
How does Product A3648, (3-Aminopropyl)triethoxysilane, compare to poly-L-lysine for binding tissue to glass surfaces?
This product has been cited as being a better tissue adhesive than poly-L-lysine. Maddox PH, Jenkins D., 3-Aminopropyltriethoxysilane (APES): a new advance in section adhesion. J Clin Pathol., 40(10):1256-7 (1987).
Can Product A3648, (3-Aminopropyl)triethoxysilane, be used to coat controlled pore glass?
To coat controlled-pore glass beads for affinity chromatography, the following method is recommended: 1. Treat 1 g of clean beads with 20 mL of a 10% aqueous A3648 (pH 3 to 4) at 75 °C for 2 hours. 2. Filter the beads to remove the solution and dry overnight in a 115 °C oven. See: J. Dairy Sci., 73, 2720-2730 (1990).
Can I buy glass slides already treated with Product A3648, (3-Aminopropyl)triethoxysilane?
Yes. Silane Prep slides, Product No. S4651, are produced using A3648. These slides have an expiration period of 24 months.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the concentration of the (3-Aminopropyl)triethoxysilane, commonly known as APTES?
The (3-Aminopropyl)triethoxysilane, which is a neat liquid, is 4.3 M. This is based on a density of 0.946 g/mL and a molecular weight of 221.37.
Can the coating made from product A3648, (3-Aminopropyl)triethoxysilane, be removed?
For Product A3648, (3-Aminopropyl)triethoxysilane, the siliconized coating can be removed by an overnight treatment with 10-20% (w/v) aqueous or alcoholic potassium hydroxide or sodium hydroxide solutions. It may also be possible to remove the coating by physical abrasion, i.e., scrubbing or vigorously brushing the glass surface. Autoclaving will not destroy the coating.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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