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A3902 Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, Vitamin B12 deficient

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Heat Shock Fractionated, Proteins and Derivatives More...
description   ≤ 1.0ug/g vitamin B12 content
assay   ≥98%
form   lyophilized powder
mol wt   mol wt ~66 kDa
purified by   heat shock fractionation
origin   USA origin
loss   ≤5%
pH   7
solubility   water: soluble (40 mg/ml)
suitability   suitable for (Suitable for use in vitamin B12 assays.)
storage temp.   2-8°C

Description

Application

For use in vitamin B12 assays.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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