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A4018 Sigma-Aldrich

Agarose, low gelling temperature

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis, Research Essentials More...
EEO   ≤0.10
mp   ≤65 °C ( at a 1.5% gel)
transition temp   congealing temperature <26-30 °C
gel strength   ≥200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.10%
foreign activity   DNase and RNase free

Description

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials. Recommended for preparation of agarose beads.

Packaging

1, 5, 10, 25, 50, 100 g in poly bottle

Quality

A low gelling temperature derivative with unique gelling properties.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Agarose Product Information

CAS Number: 9012-36-6 (product A9539) CAS Number: 39346-81-1 (products A9414, A4018, A6560, A9045, A0701) Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; ...
Keywords: Cell culture, Cloning, Counterimmunoelectrophoresis, Diffusion, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Indicators, Isoelectric focusing, Melting, Molecular biology, Polymerase chain reaction, Purification, Sample preparations, Separation

Peer-Reviewed Papers
15

References

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