A4382 Sigma-Aldrich


reagent grade

Synonym: 4-Amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one, Ampyrone



Related Categories Biochemicals and Reagents, Chemiluminescent, Detection, Enzyme Substrates, Enzymes, Inhibitors, and Substrates More...
Quality Level   PREMIUM
grade   reagent grade
mp   105-110 °C(lit.)



Reagent for glucose determination in the presence of phenol and peroxidase.


10, 25, 50 g in poly bottle

100 g in glass bottle

250 g in poly bottle

Price and Availability

Suggested Laboratory Gloves

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Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 

Protocols & Articles


Enzymatic Assay of Lipase Type XIII from Pseudomonas Species and Pseudomonas Cepacia using a coupled enzyme system of Glycerol Kinase and Glycerophosphate Oxidase (EC

1. OBJECTIVE To standardize a procedure for the enzymatic assay of Lipase Type XIII from Pseudomonas Species, Sigma Product Number L9518 , and Pseudomonas Cepacia ,Sigma Product Number L9156, using a...
Keywords: Centrifugation, Extinction coefficient, High performance liquid chromatography

Peer-Reviewed Papers


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Micro method for manual analysis of true glucose in plasma without deproteinization. Bauminger, B.B., J. Clin. Pathol. 27, 1015, (1974)


[Flow injection analysis method for hygienic examination of volatile phenol compounds in the air of residential area]. Mao L, Chen H, Liu G, et al. Wei Sheng Yan Jiu 40(6), 773-5, (2011)


Prevalence and underlying etiologies of neonatal hypoglycemia. Najati N and Saboktakin L Pak. J. Biol. Sci. 13(15), 753-6, (2010)


Coupling reaction and complex formation for the spectrophotometric determination of physiologically active catecholamines in bulk, pharmaceutical preparations and urine samples of schizophrenic patients. Nour El-Dien FA, Frag EY, and Mohamed GG Drug Test. Anal. 2(5), 234-42, (2010)


Adsorption of chromium(III), mercury(II) and lead(II) ions onto 4-aminoantipyrine immobilized bentonite. Wang Q, Chang X, Li D, et al. J. Hazard. Mater. 186(2-3), 1076-81, (2011)


The interaction between 4-aminoantipyrine and bovine serum albumin: multiple spectroscopic and molecular docking investigations. Teng Y, Liu R, Li C, et al. J. Hazard. Mater. 190(1-3), 574-81, (2011)


Novel bioactive metabolites of dipyrone (metamizol). Tobias Rogosch et al Bioorg. Med. Chem. 20, 101-7, (2012)


Diffusion of peroxides through dentine in vitro with and without prior use of a desensitizing varnish. Hannig C, Weinhold HC, Becker K, et al. Clin. Oral Investig. 15(6), 863-8, (2011)


CREBH determines the severity of sulpyrine-induced fatal shock. Kamiyama N, Yamamoto M, Saiga H, et al. PLoS ONE 8(2), e55800, (2013)


Synthesis of novel Schiff base analogues of 4-amino-1,5-dimethyl-2-phenylpyrazol-3-one and their evaluation for antioxidant and anti-inflammatory activity. Alam MS, Choi JH, and Lee DU Bioorg. Med. Chem. 20(13), 4103-8, (2012)


Enzymatic assessment of paraoxonase 1 activity on HDL subclasses: a practical zymogram method to assess HDL function. Gugliucci A, Caccavello R, Kotani K, et al. Clin. Chim. Acta 415, 162-8, (2013)


Structural, thermal, morphological and biological studies of proton-transfer complexes formed from 4-aminoantipyrine with quinol and picric acid. Adam AM Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 1-13, (2013)


Dispersive liquid-liquid microextraction combined with microvolume spectrophotometry to turn green the 5530 APHA standard method for determining phenols in water and wastewater. Lavilla I, Gil S, Costas M, et al. Talanta 98, 197-202, (2012)


Derivatization of 2-chlorophenol with 4-amino-anti-pyrine: a novel method for improving the selectivity of molecularly imprinted solid phase extraction of 2-chlorophenol from water. El-Sheikh AH, Al-Quse RW, El-Barghouthi MI, et al. Talanta 83(2), 667-73, (2010)


Synthesis, spectral characterization and biological activity of metal(II) complexes with 4-aminoantipyrine derivatives. Leelavathy C and Arul Antony S Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 113, 346-55, (2013)


Effect of 4-aminoantipyrine on oxidative stress induced by glutathione depletion in single human erythrocytes using a microfluidic device together with fluorescence imaging. Teng Y, Liu R, Li C, et al. J. Hazard. Mater. 192(3), 1766-71, (2011)


Synthesis, crystal structure, vibration spectral, and DFT studies of 4-aminoantipyrine and its derivatives. Li Y, Liu Y, Wang H, et al. Molecules 18(1), 877-93, (2013)


Exploring the DNA binding mode of transition metal based biologically active compounds. Raman N and Sobha S Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 85(1), 223-34, (2012)


Scavenging activity of aminoantipyrines against hydroxyl radical. Pedro M P Santos et al Eur. J. Med. Chem. 45, 2258-64, (2010)


Supramolecular coordination and antimicrobial activities of constructed mixed ligand complexes. El-Sonbati AZ, Diab MA, El-Bindary AA, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 213-21, (2013)


Molecular interaction between 4-aminoantipyrine and catalase reveals a potentially toxic mechanism of the drug. Teng Y, Zhang H, and Liu R Mol. Biosyst. 7(11), 3157-63, (2011)


Graphene matrix for signal enhancement in ambient plasma assisted laser desorption ionization mass spectrometry. Chang C, Li X, Bai Y, et al. Talanta 114, 54-9, (2013)


Spectroscopic and mycological studies of Co(II), Ni(II) and Cu(II) complexes with 4-aminoantipyrine derivative. Sharma AK and Chandra S Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 81(1), 424-30, (2011)


A highly selective fluorescent probe for Al3+ based on 4-aminoantipyrine. Zhou Y, Zhang J, Zhou H, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 106, 68-72, (2013)


Supramolecular and structural modification on conformational by mixed ligand. El-Sonbati AZ, Diab MA, El-Bindary AA, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 99, 211-7, (2012)


DNA binding mode of novel tetradentate amino acid based 2-hydroxybenzylidene-4-aminoantipyrine complexes. Raman N, Sobha S, Selvaganapathy M, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 96, 698-708, (2012)


High-performance liquid chromatographic assay for metamizol metabolites in rat plasma: application to pharmacokinetic studies. Domínguez-Ramírez AM, Calzadilla PC, Cortés-Arroyo AR, et al. J. Pharm. Biomed. Anal. 71, 173-8, (2012)


DNA template-assisted modulation of horseradish peroxidase activity. Datta SG, Dou X, Shibley A, et al. Int. J. Biol. Macromol. 50(3), 552-7, (2012)


Modified nanodiamonds obtained by detonation synthesis in constructing biochemical indication systems (As exemplified by the glucose determination system). Mamaeva ES, Baron AV, Puzyr AP, et al. Dokl. Biochem. Biophys. 439, 182-4, (2011)


Merck 14,591

Beil. 25,IV,3554

Aldrich MSDS 1, 58:C / Corp MSDS 1 (1), 135:D / FT-IR 1 (2), 397:A / FT-IR 2 (2), 3059:A / FT-NMR 1 (2), 1443:C / IR-Spectra (3), 1095:E / IR-Spectra (2), 956:F / NMR-Reference 2 (2), 376:A / RegBook 1 (2), 2061:L / Sigma FT-IR 1 (2), 97:B / Structure Index 1, 327:D:7

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