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A7517 Sigma-Aldrich

Bovine Serum Albumin

For use as a marker in SDS-PAGE

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

Related Categories Albumin, Albumins by Application, Biochemicals and Reagents, Bovine Serum Albumins, For Molecular Weight Markers and Protein Standards,
form   lyophilized powder
mol wt   mol wt ~66 kDa
purified by   cold ethanol fractionation
packaging   vial of 25 mg
origin   USA origin
pH   5.2
solubility   water: slightly soluble
suitability   suitable for (Suitable for use as an electrophoresis marker)
storage temp.   2-8°C

Description

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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