|Related Categories||A to C, A-AM, Bioactive Small Molecules, Biochemicals and Reagents, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes,|
|solubility||H2O: soluble50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)|
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Recommended concentrations for use are 0.1-1 mM.
Irreversible serine protease inhibitor. Inhibition constants are similar to those of PMSF and DFP. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM.
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From our library of Protocols, Sigma-Aldrich presents Chromatin Isolation by RNA Purification (ChIRP) Protocol
Keywords: Cell culture, Cell disruption, Chromatin immunoprecipitation, DNA electrophoresis, DNA purification, Electrophoresis, Gas chromatography, High performance liquid chromatography, Instructions, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, RNA purification, Sequences, Sequencing, Size-exclusion chromatography, Sonication
Irreversible enzyme inhibitors. 180. Irreversible inhibitors of the C'la component of complement derived from m-(phenoxypropoxy)benzamidine and phenoxyacetamide. Baker, B.R. and Cory, M. J. Med. Chem. 14, 119, (1971)
Markwardt, F., et al. Thromb. Res. 2, 343, (1973)
Mintz, G.R. BioPharm. 6, 34, (1993)
Chu, C., et al. J. Vis. Exp. 61, (2012)
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions. Ci C Chu, Kun K Qu, Franklin L FL Zhong, Steven E SE Artandi, Howard Y HY Chang Mol. Cell. 44, 667-78, (2011)
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