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A8654 Sigma-Aldrich

Bovine Serum Albumin

dimer mol wt ~132 kDa

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

form   lyophilized powder
mol wt   dimer mol wt ~132 kDa
  monomer mol wt ~66 kDa
  mol wt ~66 kDa
purified by   cold ethanol fractionation
origin   USA origin
pH   5.2
storage temp.   −20°C

Description

Application

Marker for non-denaturing PAGE

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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