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B2261 Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

≥98% (HPLC and TLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

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Properties

Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Biochemicals and Reagents,
Quality Level   PREMIUM
InChI Key   FYEVKHPLBHLWHK-UHFFFAOYSA-N
assay   ≥98% (HPLC and TLC)
solubility   H2O: soluble20 mg/mL
  phosphate buffer: precipitates
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic cells. It has been used for the staining of nuclei in cells, which were analyzed by flow cytometry.

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Packaging

1 g in glass bottle

100, 500 mg in glass bottle

25 mg in poly bottle

Price and Availability


Flow Cytometry Instruments
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

ABC Transporters and Cancer Drug Resistance

Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a group of...
BioFiles 2007, 2.4, 7.
Keywords: Absorption, Cancer, Clinical, Cloning, Combinatorial synthesis, Diseases, Enzymology, Gene expression, Genetic, Immunology, Pharmaceutical, Reductions, Search, Transfection, Vectors

Peer-Reviewed Papers
15

References

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