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B2883 Sigma-Aldrich

bisBenzimide H 33258

≥98% (HPLC and TLC)

Synonym: 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072

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Properties

Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Biochemicals and Reagents,
InChI Key   OWRSPPSBNWJJAR-UHFFFAOYSA-N
assay   ≥98% (HPLC and TLC)
form   powder
solubility   H2O: soluble10 mg/mL
  water and ethanol: soluble10 mg/mL
  phosphate buffer: precipitates
suitability   passes application test for fluorescence
storage temp.   −20°C

Description

Application

bisBenzimide H 33258 has been used for nuclear staining in cells.

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Packaging

1 g in glass bottle

100, 500 mg in glass bottle

25 mg in poly bottle

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SM1140500

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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