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B3801 Sigma-Aldrich

Vitamin B12 assay medium

for microbiology

Purchase

Properties

Related Categories All Food Control Media (alphabetical), All Liquid Media/Broths, Lactobacillus, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
final pH   6.1±0.2 (25 °C)
storage temp.   2-8°C
suitability   nonselective for Lactobacillus

Description

Components

Ingredients (g/L)
Casein acid hydrolysate, 15.00
Dextrose, 40.00
Asparagine, 0.20
Sodium acetate, 20.00
Ascorbic acid, 4.00
L-Cystine, 0.40
DL-Tryptophan, 0.40
Adenine sulfate, 0.02
Guanine hydrochloride, 0.02
Uracil, 0.02
Xanthine, 0.02
Riboflavin (Vitamin B2), 0.001
Thiamine hydrochloride, 0.001
Biotin, 0.00001
Niacin, 0.002
p-Amino benzoic acid, 0.002
Calcium pantothenate, 0.001
Pyridoxine hydrochloride, 0.004
Pyridoxal hydrochloride, 0.004
Pyridoxamine hydrochloride, 0.0008
Folic acid, 0.0002
Monopotassium phosphate, 1.00
Dipotassium phosphate, 1.00
Magnesium sulfate, 0.40
Sodium chloride, 0.02
Ferrous sulfate, 0.02
Manganese sulfate, 0.02
Polysorbate 80, 2.00

Application

Recommended for microbiological assay of Vitamin B12.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplement: cyanocobalamin (Cat. No. 43107)

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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