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B4501 Sigma-Aldrich

Biotin

≥99% (TLC), lyophilized powder

Synonym: D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H

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Description

Packaging

1, 5, 10, 25 g in poly bottle

100, 500 mg in poly bottle

Application

Biotin may be used to elute proteins from avidin/streptavidin resins.
• It has been used for culturing of oligodendrocytes.
• It has been used as a vitamin supplement for the growth of Bacillus species.
• It has been used for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
XJ9088200

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

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Peer-Reviewed Papers
15

References

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