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C0537 Sigma-Aldrich

5-Carboxyfluorescein

99% (HPLC)

Synonym: 5-FAM

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Properties

Related Categories C, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
InChI Key   NJYVEMPWNAYQQN-UHFFFAOYSA-N
assay   99% (HPLC)
storage temp.   2-8°C

Description

Packaging

50 mg in poly bottle

Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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