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C4916 Sigma-Aldrich

5-Carboxyfluorescein diacetate

~95% (HPLC)

Synonym: 5-CFDA, 5-Carboxy-di-O-acetylfluorescein

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Related Categories C, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
InChI Key   WPUZGNPQMIWOHE-UHFFFAOYSA-N
assay   ~95% (HPLC)
storage temp.   −20°C

Description

Packaging

100 mg in glass bottle

25 mg in poly bottle

Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem/physiol Actions

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

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Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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21879 5(6)-Carboxyfluorescein diacetate, BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

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