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D0632 Sigma-Aldrich

DL-Dithiothreitol

≥98% (HPLC), ≥99.0% (titration)

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

DTT has been used:
• as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
• as a component of medium for the demembranation and reactivation of spermatozoa.
• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.

Packaging

1 kg in poly drum

1, 5, 10, 25, 100 g in poly bottle

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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