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D3252 Sigma-Aldrich

o-Dianisidine dihydrochloride

≥95%

Synonym: 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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Properties

Related Categories Alphabetical Listing, Biochemicals and Reagents, Cell Biology, Colorimetric, D,
InChI Key   UXTIAFYTYOEQHV-UHFFFAOYSA-N
assay   ≥95%
form   powder
solubility   H2O: soluble 25 mg/mL
storage temp.   2-8°C

Description

Packaging

5, 25, 100 g in poly bottle

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Warning

Probably carcinogenic.

Physical form

crystalline

Application

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DD1050000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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