Synonym: 3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B
|Related Categories||Alphabetical Listing, Biochemicals and Reagents, Cell Biology, Colorimetric, D,|
|solubility||H2O: soluble25 mg/mL|
5, 25, 100 g in poly bottle
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
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for use with enzymatic assay kits GAGO20, STA20
tablet, 10 mg substrate per tablet
Suitable for use in glucose determination
Certificate of Analysis
Certificate of Origin
|Precautionary statements||P201-P308 + P313|
|Personal Protective Equipment||Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges|
|Hazard Codes (Europe)||T|
|Risk Statements (Europe)||45-22|
|Safety Statements (Europe)||53-45|
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
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[Mechanisms of peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate]. Kireĭko AV, Veselova IA, and Shekhovtsova TN Bioorg. Khim. 32(1), 80-6, (2006)
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Development of new methodologies for on-line determination of the bromate ion in samples of water subjected to ozonation treatment. Almendral MJ, Alonso A, and Fuentes MS J. Environ. Monit. 11(7), 1381-8, (2009)
[New approaches to the measurement of the concentration and peroxidase activity of myeloperoxidase in human blood plasma]. Gorudko IV, Cherkalina OS, Sokolov AV, et al. Bioorg. Khim. 35(5), 629-39, (2009)
Sequential and simultaneous determination of bromate and chlorite (DBPs) by flow techniques: kinetic differentiation. Alonso-Mateos A, Almendral-Parra MJ, and Fuentes-Prieto MS Talanta 76(4), 892-8, (2008)
Improved analytical sensitivity reveals the occurrence of gender-related variability in diamine oxidase enzyme activity in healthy individuals. García-Martín E, Ayuso P, Martínez C, et al. Clin. Biochem. 40(16-17), 1339-41, (2007)
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