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D5545 Sigma-Aldrich

DL-Dithiothreitol

BioXtra, ≥99.0% (titration)

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates D-F, Chemical Synthesis,
InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
Quality Level   PREMIUM
product line   BioXtra
assay   ≥99.0% (titration)
impurities   Insoluble matter, passes filter test
  ≤0.3% 4,5-dihydroxy-1,2-dithiane
pH   4.0-6.0 (20-25 °C, 0.1 m in H2O)
mp   41-44 °C(lit.)
solubility   H2O: soluble0.1 M, clear, colorless
anion traces   sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.1%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   A0.1M/260, H2O ≤0.400
  A0.1M/280, H2O ≤0.100
storage temp.   2-8°C

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Packaging

1, 5, 25 g in glass bottle

Price and Availability


Laboratory Gloves

Amplified Detection
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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