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D8001 Sigma-Aldrich

3,3′-Diaminobenzidine

≥99% (HPLC)

Synonym: 3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB

  • CAS Number 91-95-2

  • Linear Formula (NH2)2C6H3C6H3(NH2)2

  • Molecular Weight 214.27

  •  Beilstein Registry Number 1212988

  •  EC Number 202-110-6

  •  MDL number MFCD00007725

  •  PubChem Substance ID 24894175

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Description

Application

DAB (3,3′-Diaminobenzidine) has been used in the immunohistological staining procedure.

Peroxidase substrate; reagent for spectrophotometric determination of selenium.

Packaging

1, 5 g in glass bottle

10, 25 g in poly bottle

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
DV8750000
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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