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D8001 Sigma-Aldrich

3,3′-Diaminobenzidine

≥99% (HPLC)

Synonym: 3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB

  • CAS Number 91-95-2

  • Linear Formula (NH2)2C6H3C6H3(NH2)2

  • Molecular Weight 214.27

  •  Beilstein Registry Number 1212988

  •  EC Number 202-110-6

  •  MDL number MFCD00007725

  •  PubChem Substance ID 24894175

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Description

Application

DAB (3,3′-Diaminobenzidine) has been used in the immunohistological staining procedure.

Peroxidase substrate; reagent for spectrophotometric determination of selenium.

Packaging

1, 5 g in glass bottle

10, 25 g in poly bottle

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.

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Laboratory Gloves

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
DV8750000
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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