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D8284 Sigma-Aldrich

Bicinchoninic acid disodium salt hydrate

≥98% (HPLC)

Synonym: 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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Properties

Related Categories General Protein Quantitation, Molecular Biology, Protein Quantitation, Proteomics, Quantitation Kits and Reagents More...
InChI Key   FAFVGCAYKZKOPT-UHFFFAOYSA-L
assay   ≥98% (HPLC)
form   powder
color   white to light yellow
solubility   0.5% sodium bicarbonate: soluble50 mg/mL
storage temp.   2-8°C

Description

Packaging

1, 5, 10, 25 g in poly bottle

Application

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
• It has been used for determining protein concentration.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




Amplified Detection

Direct Detect Infrared Spectrometer
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Related Products

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Description

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QPBCA QuantiPro BCA Assay Kit, for 0.5-30 μg/ml protein
BCA1 Bicinchoninic Acid Kit for Protein Determination, for 200-1000 μg/ml protein
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