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D8284 Sigma-Aldrich

Bicinchoninic acid disodium salt hydrate

≥98% (HPLC)

Synonym: 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt, 4,4′-Dicarboxy-2,2′-biquinoline disodium salt, BCA, Disodium 2,2′-biquinoline-4,4′-dicarboxylate

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Properties

Related Categories General Protein Quantitation, Molecular Biology, Protein Quantitation, Proteomics, Quantitation Kits and Reagents More...
InChI Key   FAFVGCAYKZKOPT-UHFFFAOYSA-L
assay   ≥98% (HPLC)
form   powder
color   white to light yellow
solubility   0.5% sodium bicarbonate: 50 mg/mL
storage temp.   2-8°C

Description

Packaging

1, 5, 10, 25 g in poly bottle

Application

• Bicinchoninic acid disodium salt hydrate has been used to monitor the levels of reducing sugars, thereby helping in the detection of chitinases.
• It has also been used to determine the amount of cellulose reducing ends (micromoles/g cellulose).
• It has been used for determining protein concentration.

Biochem/physiol Actions

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+), in presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Price and Availability


Laboratory Gloves

Amplified Detection
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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QPBCA QuantiPro BCA Assay Kit, for 0.5-30 μg/ml protein
BCA1 Bicinchoninic Acid Kit for Protein Determination, for 200-1000 μg/ml protein
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