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E6383 Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

crystalline

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride, EDC, WSC hydrochloride

Properties

Related Categories Crosslinking, Heterobifunctional Cross-Linking Reagents, Molecular Biology, Protein Modification, Protein Structural Analysis,
Quality Level   PREMIUM
form   crystalline
mp   110-115 °C(lit.)
solubility   H2O: ≤100 mg/mL
storage temp.   −20°C

Description

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

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