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E7750 Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

commercial grade, powder

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride, EDC, WSC hydrochloride

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Properties

Related Categories Crosslinking, Heterobifunctional Cross-Linking Reagents, Molecular Biology, Protein Modification, Protein Structural Analysis,
Quality Level   PREMIUM
grade   commercial grade
InChI Key   FPQQSJJWHUJYPU-UHFFFAOYSA-N
form   powder
mp   110-115 °C(lit.)
solubility   H2O: ≤100 mg/mL
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 5 kg in poly drum

1, 5, 10, 25, 100 g in poly bottle

100 mg in poly bottle

Application

• N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for the formation of FND (fluorescent nanodiamonds)-transferrin bioconjugates.
• It has been used for crosslinking polyethylenimine to gold particles.
• It has been used as a carbodiimide linkage agent for coating of carboxylated polystyrene beads with biotinylated BSA (bovine serum albumin).

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

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Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
FF2200000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Product No. E7750, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, is listed as “commercial grade”. What does that mean?
It means that it is the grade of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDAC) hydrochloride that was commercially available at the time we purchased it. This product is not assayed for purity. If you need a high purity EDAC, consider Product No. 03450.
I understand that E7750, N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, can be used in peptide synthesis. Do you have a brochure on some other reagents that can be used for peptide synthesis?
Yes. Please see: http://www.sigmaaldrich.com/aldrich/brochure/al_chemfile_v7_n2.pdf
Can N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, Product E7750, be used as a peptide coupling reagent in alcoholic solvents? If so, do you have a reference?
Yes. Nosaki, S., Effects of amounts of additives on peptide coupling mediated by a water-soluble carbodiimide in alcohols. J. Peptide Res., 54(2), 162-167 (1999). This paper describes using the E7750 along with 3,4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine (HOOBt) and 1-hydroxy-7-azabenzotriazole (HOAt) for enhancement of peptide coupling.
What impurities might be present in N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, Product E7750?
The two compounds most likely to appear in EDAC as trace impurities are 3-dimethylaminopropylamine and N-(3-dimethylaminopropyl)-N'-ethylurea as a result of synthesis (intermediate) and degradation (moisture or coupling reaction), respectively.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

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Keywords: Building blocks, Catalysis, Cycloadditions, Esterifications, Materials Science, Nucleophilic substitutions, Polymer science, Polymerization reactions, Radical polymerization, Substitutions, transformation

Peer-Reviewed Papers
15

References

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