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E8751 Sigma-Aldrich

Ethidium bromide

~95% (HPLC)

Synonym: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Packaging

1, 5, 10, 25 g in glass bottle

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811PIH 6.1 / PGI
WGK Germany 
3
RTECS 
SF7950000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the best working concentration of Product E8751, Ethidium bromide?
For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/mL with water and incubate the gel for 15-30 min.  Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL, and visualized immediately after electrophoresis.
How can I make a stock solution of Product E8751, Ethidium bromide?
Ethidium bromide is soluble in water at 100 mg/mL. Per Crary, B. and Boyrsenko, M., Biotechniques, 4, 98-101 (1986), stock solutions of ethidium bromide in water or PBS are stable for at least two years at room temperature if protected from light.
How can I dispose of solutions containing Product E8751, Ethidium bromide?
Ethidium bromide is mutagenic, and ultimately has to be destroyed by incineration. It can be removed from buffer solutions by several methods to reduce the volume of waste. See the product sheet for more details: http://www.sigmaaldrich.com/sigma-aldrich/product_information_sheet/e8751pis.pdf
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Ethidium Bromide

Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide; 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; Homidium Bromide; Dromilac; EtBr
Keywords: Absorption, Electrophoresis, Gel electrophoresis, Melting, Molecular biology, Veterinary

Peer-Reviewed Papers
15

References

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