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ED2SS Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2




Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.


1 kg in poly bottle

2.5, 5 kg in poly drum

50, 100, 250, 500 g in poly bottle

Biochem/physiol Actions

Zinc-dependent metalloproteinases, as well as other proteases that are stabilized by calcium, can be effectively inhibited by chelation of divalent metal ions with EDTA. Other chelators such as EGTA, specific for calcium, and 1,10-phenanthroline, highly specific for zinc, can be used to target these two different types of proteases.

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Determination of Water Content in EDTA disodium salt dihydrate Using Karl Fischer Titration

Group(s): Nitrogen Compounds, Pharmaceutical Products + Compounds, Cosmetic Products + Compounds
Keywords: Pharmaceutical, Titrations

Determination of Water Content in Trilon BD Using Karl Fischer Titration

Cosmetic products are very often preparations with a very high water content which must be exactly determined. This requires precise sampling, weighing and administration of the substance. The sample...
Keywords: Sample preparations, Titrations


Enzymatic Assay and ATP Sensitivity Test of Luciferase

To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Keywords: Biochemistry, Enzyme activity, Enzymology, Size-exclusion chromatography

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Superoxide Dismutase

This procedure applies to all products that have a specification for superoxide dismutase activity by enzymatic determination.
Keywords: Catalysis, Reductions

Peer-Reviewed Papers


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