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ED2SS Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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Description

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Packaging

1 kg in poly bottle

2.5, 5 kg in poly drum

50, 100, 250, 500 g in poly bottle

Biochem/physiol Actions

Zinc-dependent metalloproteinases, as well as other proteases that are stabilized by calcium, can be effectively inhibited by chelation of divalent metal ions with EDTA. Other chelators such as EGTA, specific for calcium, and 1,10-phenanthroline, highly specific for zinc, can be used to target these two different types of proteases.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



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Edetate disodium

meets USP testing specifications

Ethylenediaminetetraacetic acid disodium salt dihydrate

for electrophoresis, for molecular biology, 99.0-101.0% (titration)

Ethylenediaminetetraacetic acid disodium salt dihydrate

Sigma Grade, plant cell culture tested, 98.5-101.5%

Ethylenediaminetetraacetic acid disodium salt dihydrate

meets analytical specification of Ph. Eur., BP, USP, FCC, 99.0-101.0%

Safety & Documentation

Safety Information

WGK Germany 
2
RTECS 
AH4410000
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C

Protocols & Articles

Articles

Determination of Water Content in EDTA disodium salt dihydrate Using Karl Fischer Titration

Group(s): Nitrogen Compounds, Pharmaceutical Products + Compounds, Cosmetic Products + Compounds
Keywords: Pharmaceutical, Titrations

Determination of Water Content in Trilon BD Using Karl Fischer Titration

Cosmetic products are very often preparations with a very high water content which must be exactly determined. This requires precise sampling, weighing and administration of the substance. The sample...
Keywords: Sample preparations, Titrations

Protocols

Enzymatic Assay and ATP Sensitivity Test of Luciferase

1. OBJECTIVE To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Keywords: Biochemistry, Enzyme activity, Enzymology, Size-exclusion chromatography

Enzymatic Assay of Choloylglycine Hydrolase

1. OBJECTIVE To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Superoxide Dismutase

1. OBJECTIVE To standardize a procedure for the enzymatic determination of superoxide dismutase.
Keywords: Catalysis, Reductions

Peer-Reviewed Papers

References

Set your institution to view full text papers.

High sensitive gold-nanoparticle based lateral flow Immunodevice for Cd2+ detection in drinking waters. López Marzo AM, Pons J, Blake DA, et al. Biosens. Bioelectron. 47, 190-8, (2013)

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Iron bioavailability from a lipid-based complementary food fortificant mixed with millet porridge can be optimized by adding phytase and ascorbic acid but not by using a mixture of ferrous sulfate and sodium iron EDTA. Cercamondi CI, Egli IM, Mitchikpe E, et al. J. Nutr. 143(8), 1233-9, (2013)

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Most blood biomarkers related to vitamin status, one-carbon metabolism, and the kynurenine pathway show adequate preanalytical stability and within-person reproducibility to allow assessment of exposure or nutritional status in healthy women and cardiovascular patients. Midttun O, Townsend MK, Nygård O, et al. J. Nutr. 144(5), 784-90, (2014)

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The effect of amniotic membrane de-epithelialization method on its biological properties and ability to promote limbal epithelial cell culture. Zhang T, Yam GH, Riau AK, et al. Invest. Ophthalmol. Vis. Sci. 54(4), 3072-81, (2013)

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Master file size and apical transportation in severely curved root canals. Moradi S, Disfani R, Baziar H, et al. N. Z. Dent. J. 110(4), 126-9, (2014)

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Impact of cadmium, cobalt and nickel on sequence-specific DNA binding of p63 and p73 in vitro and in cells. Adámik M, Bažantová P, Navrátilová L, et al. Biochem. Biophys. Res. Commun. 456(1), 29-34, (2015)

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Effect of irrigation with Tetraclean on bacterial leakage of obturated root canals. Mohammadi Z, Shalavi S, Giardino L, et al. N. Y. State Dent. J. 80(3), 39-43, (2014)

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Intracellular MMP-2 activity in skeletal muscle is associated with type II fibers. Hadler-Olsen E, Solli AI, Hafstad A, et al. J. Cell Physiol. 230(1), 160-9, (2015)

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The role of endodontics in interdisciplinary dentistry: are you making the right decisions? West J Dent. Today 33(4), 80, 82, 84-5, (2014)

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[Removal of heavy metals from extract of Angelica sinensis by EDTA-modified chitosan magnetic adsorbent]. Ren Y, Sun MH, Peng H, et al. Zhongguo Zhong Yao Za Zhi 38(21), 3709-12, (2013)

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Effect of maleic acid and ethylenediaminetetraacetic acid on the shear bond strength of RealSeal SE sealer to root canal dentin. Ballal NV, Tweeny A, Baumgartner JC, et al. Eur. J. Prosthodont. Restor. Dent. 21(4), 152-6, (2013)

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[73-year-old man with refractory idiopathic thrombocytopenia purpura]. Austein T and Lehmann J Dtsch. Med. Wochenschr. 138(46), 2357-8, (2013)

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[Membrane-bound proteases of ompT+ and ompT- Vibrio cholerae strains]. Kozlov SN, Nikolaev VB, Markov EIu, et al. Zh. Mikrobiol. Epidemiol. Immunobiol. (2), 3-12, (2013)

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[Alternative medicine sensation. Surprisingly effective: chelates and multivitamins in coronary heart disease]. Einecke D MMW Fortschr. Med. 155(6), 23, (2013)

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Effect of three different contamination removal methods on bond strength of a self-etching adhesive to dentin contaminated with an aluminum chloride hemostatic agent. Ajami AA, Kahnamoii MA, Kimyai S, et al. J. Contemp. Dent. Pract. 14(1), 26-33, (2013)

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Chelation therapy may improve cardiovascular health. BMJ 346, f2071, (2013)

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ProTaper rotary instrument fracture during root canal preparation: a comparison between rotary and hybrid techniques. Farid H, Khan FR, and Rahman M Oral Health Dent. Manag. 12(1), 50-5, (2013)

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Facile preparation of glutathione-stabilized gold nanoclusters for selective determination of chromium (III) and chromium (VI) in environmental water samples. Zhang H, Liu Q, Wang T, et al. Anal. Chim. Acta 770, 140-6, (2013)

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Treatment of oxaliplatin-induced peripheral neuropathy by intravenous mangafodipir. Coriat R, Alexandre J, Nicco C, et al. J. Clin. Invest. 124(1), 262-72, (2014)

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Effect of disodium EDTA chelation regimen on cardiovascular events in patients with previous myocardial infarction: the TACT randomized trial. Lamas GA, Goertz C, Boineau R, et al. JAMA 309(12), 1241-50, (2013)

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Simulation of the effect of maize porridge fortified with grain amaranth or micronutrient powder containing NaFeEDTA on iron intake and status in Kenyan children. Macharia-Mutie CW, Omusundi AM, Mwai JM, et al. Publ. Health Nutr. 16(9), 1605-13, (2013)

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Brand of dipotassium EDTA vacuum tube as a new source of pre-analytical variability in routine haematology testing. Lima-Oliveira G, Lippi G, Salvagno GL, et al. Br. J. Biomed. Sci. 70(1), 6-9, (2013)

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The HIV-2 Rev-response element: determining secondary structure and defining folding intermediates. Lusvarghi S, Sztuba-Solinska J, Purzycka KJ, et al. Nucleic Acids Res. 41(13), 6637-49, (2013)

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Rat pancreatitis produced by 13-week administration of zinc oxide nanoparticles: biopersistence of nanoparticles and possible solutions. Seok SH, Cho WS, Park JS, et al. J. Appl. Toxicol. 33(10), 1089-96, (2013)

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Separation of digesta fractions complicates estimation of ileal digestibility using marker methods with Cr2O3 and cobalt-ethylenediamine tetraacetic acid in broiler chickens. de Vries S, Kwakkel RP, Pustjens AM, et al. Poult. Sci. 93(8), 2010-7, (2014)

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Reclamation of copper-contaminated soil using EDTA or citric acid coupled with dissolved organic matter solution extracted from distillery sludge. Liu CC and Lin YC Environ. Pollut. 178, 97-101, (2013)

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Influence of the selective EDTA derivative phenyldiaminetetraacetic acid on the speciation and extraction of heavy metals from a contaminated soil. Zhang T, Wei H, Yang XH, et al. Chemosphere 109, 1-6, (2014)

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Phenotypic analysis of Eschericia coli mutants lacking L,D-transpeptidases. Sanders AN and Pavelka MS Microbiology 159(Pt 9), 1842-52, (2013)

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EDTA chelation therapy alone and in combination with oral high-dose multivitamins and minerals for coronary disease: The factorial group results of the Trial to Assess Chelation Therapy. Lamas GA, Boineau R, Goertz C, et al. Am. Heart J. 168(1), 37-44.e5, (2014)

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Zinc is released by cultured astrocytes as a gliotransmitter under hypoosmotic stress-loaded conditions and regulates microglial activity. Segawa S, Nishiura T, Furuta T, et al. Life Sci. 94(2), 137-44, (2014)

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In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium. Aihara E, Hentz CL, Korman AM, et al. J. Biol. Chem. 288(47), 33585-97, (2013)

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Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein. Hirata A, Hori Y, Koga Y, et al. BMC Biotechnol. 13, 19, (2013)

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La-EDTA coated Fe3O4 nanomaterial: preparation and application in removal of phosphate from water. Yang J, Zeng Q, Peng L, et al. J. Environ. Sci. (China) 25(2), 413-8, (2013)

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Novel lipoprotein density profiling in healthy dogs of various breeds, healthy Miniature Schnauzers, and Miniature Schnauzers with hyperlipidemia. Xenoulis PG, Cammarata PJ, Walzem RL, et al. BMC Vet. Res. 9, 47, (2013)

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Oxidative damage of rat liver mitochondria during exposure to t-butyl hydroperoxide. Role of Ca²⁺ ions in oxidative processes. Zavodnik IB, Dremza IK, Cheshchevik VT, et al. Life Sci. 92(23), 1110-7, (2013)

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Effects of Mn-DPDP and manganese chloride on hemodynamics and glucose tolerance in anesthetized rats. Gao X, Sandberg M, Quach M, et al. Acta radiol. 55(3), 328-34, (2014)

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Sulfate removal by Desulfovibrio sp. CMX in chelate scrubbing solutions for NO removal. Chen M, Zhang Y, Zhou J, et al. Bioresour. Technol. 143, 455-60, (2013)

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Ethylenediaminetetraacetic acid-dependent pseudomacrocytosis. Vagace JM, Rodriguez MÁ, de la Maya MD, et al. J. Clin. Pathol. 66(9), 811-4, (2013)

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Matrix metalloproteinase-2 (MMP-2) generates soluble HLA-G1 by cell surface proteolytic shedding. Rizzo R, Trentini A, Bortolotti D, et al. Mol. Cell Biochem. 381(1-2), 243-55, (2013)

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Theoretical and experimental studies on isotachophoresis in multi-moving chelation boundary system formed with metal ions and EDTA. Zhang W, Guo CG, Fan LY, et al. Analyst 138(17), 5039-51, (2013)

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Chelant soil-washing technology for metal-contaminated soil. Voglar D and Lestan D Environ. Technol. 35(9-12), 1389-400, (2014)

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Determination of vanadium in groundwater samples with an improved kinetic spectrophotometric method. Bağda E Environ. Technol. 35(9-12), 1165-74, (2014)

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Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region. Tiyanont K, Wales TE, Siebel CW, et al. J. Mol. Biol. 425(17), 3192-204, (2013)

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The regulation of apoptosis by the downstream regulatory element antagonist modulator/potassium channel interacting protein 3 (DREAM/KChIP3) through interactions with hexokinase I. Craig TA, Ramachandran PL, Bergen HR, et al. Biochem. Biophys. Res. Commun. 433(4), 508-12, (2013)

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Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt. Schuff-Werner P, Steiner M, Fenger S, et al. Br. J. Haematol. 162(5), 684-92, (2013)

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Fortification of wheat flour and maize meal with different iron compounds: results of a series of baking trials. Randall P, Johnson Q, and Verster A Food Nutr. Bull. 33(4 Suppl), S344-59, (2012)

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Effect of EDTA and citric acid on phytoremediation of Cr- B[a]P-co-contaminated soil. Chigbo C and Batty L Environ. Sci. Pollut. Res. Int. 20(12), 8955-63, (2013)

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Zn(2+) rather than Ca(2+) or Mg(2+) used as a cofactor in non-muscular actin from the oyster to control protein polymerization. Yang X, Lv C, Zhang S, et al. Biochim. Biophys. Acta 1830(8), 4179-88, (2013)

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Modified Fenton oxidation of diesel fuel in arctic soils rich in organic matter and iron. Sherwood MK and Cassidy DP Chemosphere 113, 56-61, (2014)

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Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Chu Q, Liu L, and Wang W Cell Prolif. 46(3), 254-62, (2013)

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Performance evaluation of Samsung LABGEO(HC10) Hematology Analyzer. Park IJ, Ahn S, Kim YI, et al. Arch. Pathol. Lab. Med. 138(8), 1077-82, (2014)

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Redox state of p63 and p73 core domains regulates sequence-specific DNA binding. Tichý V, Navrátilová L, Adámik M, et al. Biochem. Biophys. Res. Commun. 433(4), 445-9, (2013)

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Safety and efficacy of ethylenediaminetetraacetic acid for removing microcapsules. Ren M, Liu R, Lv B, et al. J. Surg. Res. 183(1), 442-9, (2013)

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Separation of metals and phosphorus from incinerated sewage sludge ash. Ito A, Yamada K, Ishikawa N, et al. Water Sci. Technol. 67(11), 2488-93, (2013)

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Novel thrombolytic protease from edible and medicinal plant Aster yomena (Kitam.) Honda with anticoagulant activity: purification and partial characterization. Choi JH, Kim DW, Park SE, et al. J. Biosci. Bioeng. 118(4), 372-7, (2014)

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Percentage exposure of root dentin collagen after application of two irrigation protocols with manual or rotary instrumentation and two methacrylate resin-based sealers. González-López S, Martín-Altuve E, Bolaños-Carmona V, et al. J. Adhes. Dent. 15(5), 481-9, (2013)

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Tissue- and cell-specific co-localization of intracellular gelatinolytic activity and matrix metalloproteinase 2. Solli AI, Fadnes B, Winberg JO, et al. J. Histochem. Cytochem. 61(6), 444-61, (2013)

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The sequential use of washing and an electrochemical reduction process for the remediation of lead-contaminated soils. Demir A and Köleli N Environ. Technol. 34(5-8), 799-805, (2013)

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EDTA-enhanced phytoremediation of contaminated calcareous soils: heavy metal bioavailability, extractability, and uptake by maize and sesbania. Suthar V, Memon KS, and Mahmood-ul-Hassan M Environ. Monit. Assess. 186(6), 3957-68, (2014)

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The effectiveness and risk comparison of EDTA with EGTA in enhancing Cd phytoextraction by Mirabilis jalapa L. Wang S and Liu J Environ. Monit. Assess. 186(2), 751-9, (2014)

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Monitoring the effects of chelating agents and electrical fields on active forms of Pb and Zn in contaminated soil. Tahmasbian I and Safari Sinegani AA Environ. Monit. Assess. 185(11), 8847-60, (2013)

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In vitro activity of novel in silico-developed antimicrobial peptides against a panel of bacterial pathogens. Romani AA, Baroni MC, Taddei S, et al. J. Pept. Sci. 19(9), 554-65, (2013)

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EDTA-dependent pseudothrombocytopenia complicated by eosinophilic pneumonia. Ohashi N, Nakamura K, Inokuchi R, et al. Am. J. Emerg. Med. 31(7), 1157.e5-7, (2013)

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GidA expression in Salmonella is modulated under certain environmental conditions. Rehl JM, Shippy DC, Eakley NM, et al. Curr. Microbiol. 67(3), 279-85, (2013)

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Photocatalytic removal of selenite and selenate species: effect of EDTA and other process variables. Labaran BA and Vohra MS Environ. Technol. 35(9-12), 1091-100, (2014)

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Trial to Assess Chelation Therapy (TACT) and equipoise: When evidence conflicts with beliefs. Maron DJ and Hlatky MA Am. Heart J. 168(1), 4-5, (2014)

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The search for treatments to reduce chemotherapy-induced peripheral neuropathy. Pachman DR, Loprinzi CL, Grothey A, et al. J. Clin. Invest. 124(1), 72-4, (2014)

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Pseudothrombocytopenia in a patient receiving romiplostim for immune thrombocytopenic purpura. Fozza C, Pardini S, Marras T, et al. Ann. Hematol. 93(5), 899-900, (2014)

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Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances. Dingle TC, Sedlak RH, Cook L, et al. Clin. Chem. 59(11), 1670-2, (2013)

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Chelation therapy and cardiovascular outcomes. Kleinman B JAMA 310(4), 431, (2013)

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Chelation therapy and cardiovascular outcomes. Bittl JA JAMA 310(4), 430-1, (2013)

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Concerns about reliability in the Trial to Assess Chelation Therapy (TACT). Nissen SE JAMA 309(12), 1293-4, (2013)

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Evaluation of the Trial to Assess Chelation Therapy (TACT): the scientific process, peer review, and editorial scrutiny. Bauchner H, Fontanarosa PB, and Golub RM JAMA 309(12), 1291-2, (2013)

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Merck 14,3517

Beil. 4,IV,2451

Corp MSDS 1 (1), 1606:D / FT-IR 2 (1), 902:B / FT-IR 1 (1), 567:B / IR-Spectra (2), 303:G / IR-Spectra (3), 340:D / RegBook 1 (1), 647:I / Sigma FT-IR 1 (2), 549:B / Structure Index 1, 94:A:8

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