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F5415 Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

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Properties

Related Categories Cell Biology, Centrifugation Media, Core Bioreagents, Density Gradient Media, General Stem Cell Biology,
sterility   0.2 μm filtered
type   Type 400
concentration   20% in H2O
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of GE Healthcare.

Ficoll is a registered trademark of GE Healthcare

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?
No.  The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL.  Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL.  Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical.  Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.
Is Product F5415, Ficoll® solution 20%, sterile?
No claims are made for sterility for F5415.  If a sterile product is required for cell culture use,  product F8636 has been gamma irradiated and is considered a sterile product.  Product F8636 is a powder and does not come as a sterile solution.
What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?
Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark.  Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.
Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?
This is not recommended.  Ficoll has a molecular weight in the range of 400,000.  Even 20% solutions of such high molecular weight products have extremely low osmolality.  Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic.  Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.
Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?
No.  However there is a Product Information Sheet for product F4375.  This product information sheet gives general information regarding Ficoll and possible uses.  If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.
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Protocols & Articles
Peer-Reviewed Papers
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