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F5422 Sigma-Aldrich

Folic acid assay medium

for microbiology



Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Media, Media by Application,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
pH   6.8±0.2 (25 °C)
storage temp.   2-8°C
suitability   nonselective for Streptococcus



Components (g/L)
Casein Acid Hydrolysate, Vitamin Free 12.00
Dextrose 40.00
Sodium Citrate 20.00
L-Cystine 0.20
DL-Tryptophan 0.20
Adenine Sulfate 0.02
Guanine Hydrochloride 0.02
Uracil 0.02
Thiamine Hydrochloride 0.002
Pyridoxine Hydrochloride 0.004
Riboflavin (Vitamin B2) 0.002
Nacin 0.002
p-Aminobenzoic Acid (PABA) 0.0002
Biotin 0.0000008
Calcium Pantothenate 0.0004
Dipotassium Phosphate 1.00
Monopotassium Phosphate 1.00
Magnesium Sulfate 0.40
Sodium Chloride 0.02
Ferrous Sulphate 0.02
Manganese Sulphate 0.02


Recommended for the microbiological assay of folic acid using Streptococcus faecium as the test organism.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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