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F7258 Sigma-Aldrich

Fast Green FCF

certified by the Biological Stain Commission

Synonym: Food green 3

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, E-F, Hematology and Histology,
grade   certified by the Biological Stain Commission
InChI Key   RZSYLLSAWYUBPE-UHFFFAOYSA-L
form   powder
composition   dye content, ≥90%
mp   290 °C (dec.)(lit.)
solubility   H2O: soluble1 mg/mL

Description

Application

Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

25 g in glass bottle

Suitability

Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.

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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
BQ4425000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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